Production of Recombinant Proteins

Filling the gap created over the past five years, during which many new techniques have entered the market, this book is directed at both the new and the experienced ion channel researcher wishing to learn more about the considerations and methods for studying recombinant ion channels. These latest developments are covered here for the first time, contributed by editors and authors working for major pharmaceutical companies and who routinely apply these techniques in their daily work. The first three chapters cover the use of the Xenopus oocyte expression system for structure-function studies, from basic approaches for manipulating ion channel cDNAs to more specialized but powerful techniques. This is followed by reviews of strategies and methodologies available for expressing channels in mammalian cells and for their analysis by patch-clamp electrophysiology. Chapters 6 to 8 review the latest methodologies for ion channel drug discovery, including high throughput screening using fluorescence and luminescence, as well as automated planar array electrophysiology. The remaining two chapters focus on approaches for determining ion channel crystal structures and on computational approaches to understanding channel mechanisms at atomic resolution. Rather than provide detailed protocols, indicated by references in each chapter, the authors provide a comprehensive and easily accessible overview of the techniques involved, reviewing underlying principles and providing working guidelines as well as an understanding of the key theoretical and practical considerations associated with each topic. In each case, this practical advice is illustrated by real life examples, taken either from the author's own experience or from key examples in the literature, providing valuable practical hints not found elsewhere. The result is a compendium of practical ion channel information that will prove a valuable resource to academic and industrial workers alike.

Meeting the need for a book on developing and using new methods to investigate membrane proteins, this is the first of its kind to present the full range of novel techniques in one resource. Top researchers from around the world focus on the physical principles exploited in the different techniques, and provide examples of how these can bring about important new insights. Following an introduction, further sections discuss structural approaches, molecular interaction and large assemblies, dynamics and spectroscopies, finishing off with an exploration of structure-function relationships in whole cells.

This reference work provides a comprehensive insight into past developments in the application of non-linear dynamics, such as production systems in the manufacturing and process engineering, mechanical engineering and plant construction and automation technology. As such, it is the first publication to document the successful implementation of non-linear dynamics into current tasks or problems of engineering thus far unsolved. The interdisciplinary team of contributors from research and industry establishes ties between mechanical methods of manufacturing and new methods reaching the dynamics of production lines and complete production systems.

In this first book dedicated to the logistics of chemical plants and production processes, authors from academia and industry – such as Bayer, Degussa, Merck – provide an overview of the field, incorporating the knowledge and experience gathered over the last 10 years. In so doing, they describe the latest ideas on efficient design, illustrating when to produce which part of the equipment and with which resources, so as to optimize chemical plants for high capacity and flexibility. This book gives an overview of the state-of-the-art of the whole logistic chain of chemical production processes. Alongside the fundamentals, tools and algorithms, and integration issues, the book features five significant industrial case studies.

Presenting a wide-ranging view of current developments in protein research, the papers in this collection, each written by highly regarded experts in the field, examine various aspects of protein structure, functions, dynamics, and experimentation. Topics include dynamical simulation methods, the biological role of atom fluctuations, protein folding, influences on protein dynamics, and a variety of analytical techniques, such as X-ray diffraction, vibrational spectroscopy, photodissociation and rebinding kinetics. This is part of a series devoted to providing general information on a wide variety of topics in chemical physics in order to stimulate new research and to serve as a text for beginners in a particular area of chemical physics.

This book describes medical applications of recombinant proteins and monoclonal antibodies, some of which have already been on the market for several years while others have only recently been launched. It also highlights the manufacturing processes for individual products, the strategies that were taken by companies in the clinical development, and the hurdles that were encountered in clinical trials and had to be overcome before approval by regulatory authorities. Finally, this book illustrates strategies to modify and improve the pharmacodynamic and pharmacokinetic properties of naturally occurring proteins thus paving the way for a new era in biotechnology. Foreword written by Jurgen Drews.

Oil and gas still power the bulk of our world, from automobiles and the power plants that supply electricity to our homes and businesses, to jet fuel, plastics, and many other products that enrich our lives. With the relatively recent development of hydraulic fracturing («fracking»), multilateral, directional, and underbalanced drilling, and enhanced oil recovery, oil and gas production is more important and efficient than ever before. Along with these advancements, as with any new engineering process or technology, come challenges, many of them environmental. More than just a text that outlines the environmental challenges of oil and gas production that have always been there, such as gas migration and corrosion, this groundbreaking new volume takes on the most up-to-date processes and technologies involved in this field. Filled with dozens of case studies and examples, the authors, two of the most well-known and respected petroleum engineers in the world, have outlined all of the major environmental aspects of oil and gas production and how to navigate them, achieving a more efficient, effective, and profitable operation. This groundbreaking volume is a must-have for any petroleum engineer working in the field, and for students and faculty in petroleum engineering departments worldwide.

The science and practice of pig production has changed rapidly over recent decades; new husbandry practices, new understandings of growth, reproduction and health, new appreciations of welfare and environmental impact, new nutritional approaches, and modern reproductive and genetic techniques have all come into being, together with the emergence of new health challenges. Now in its third edition, this long established reference book on the management, breeding, feeding, nutrition, health and welfare of pigs has been fully revised to provide clear and current information on both the practical and scientific aspects of the pig industry. With the help of a new panel of international experts and a senior editor, the overall structure now contains input from international centres across Europe and North America. This edition includes: Updated versions of existing chapters; Completely revised and new sections on: Pig meat and carcass quality, Reproduction, The maintenance of health, Nutritional value of protein and amino acids in feed stuffs, Value of fats and oils in pig diets, Product marketing, Environmental management, Simulation modelling; Input from international authorities; Many tables, diagrams, photographs and figures.

The performance of an company depends both on its technological expertise and its managerial and organizational effectiveness. Production management is an important part of the process for manufacturing firms. The organization of production relies in general on the implementation of a certain number of basic functions, among which the scheduling function plays an essential role. This title presents recently developed methods for resolving scheduling issues. The basic concepts and the methods of production scheduling are introduced and advanced techniques are discussed, providing readers with a comprehensive and accessible guide to employing this process.

With its integral treatment of ecosystem and resource management, this is the only overview of the field to address current thinking and future trends. All contributions have been written with the novice in mind, explaining the basics and highlighting recent developments and achievements. Unmatched in scope, this two-volume reference covers both traditional and well-established areas of marine biotechnology, such as biomass production, alongside such novel ones as biofuels, biological protection of structures and bioinspired materials. In so doing, it ties together information usually only found in widely dispersed sources to assemble a grand unified view of the current state of and prospects for this multi-faceted discipline. The combination of the breadth of topics and the focus on modern ideas make this introductory book especially suitable for teaching purposes and for guiding newcomers to the many possibilities offered by this booming field.