Advances in Acoustic Microscopy and High Resolution Imaging

This unique book on super-resolution microscopy techniques presents comparative, in-depth analyses of the strengths and weaknesses of the individual approaches. It was written for non-experts who need to understand the principles of super-resolution or who wish to use recently commercialized instruments as well as for professionals who plan to realize novel microscopic devices. Explaining the practical requirements in terms of hardware, software and sample preparation, the book offers a wealth of hands-on tips and practical tricks to get a setup running, provides invaluable help and support for successful data acquisition and specific advice in the context of data analysis and visualization. Furthermore, it addresses a wide array of transdisciplinary fields of applications. The author begins by outlining the joint efforts that have led to achieving super-resolution microscopy combining advances in single-molecule photo-physics, fluorophore design and fluorescent labeling, instrument design and software development. The following chapters depict and compare current main standard techniques such as structured illumination microscopy, single-molecule localization, stimulated emission depletion microscopy and multi-scale imaging including light-sheet and expansion microscopy. For each individual approach the experimental setups are introduced, the imaging protocols are provided and the various applications illustrated. The book concludes with a discussion of future challenges addressing issues of routine applications and further commercialization of the available methods. Guiding users in how to make choices for the design of their own experiments from scratch to promising application, this one-stop resource is intended for researchers in the applied sciences, from chemistry to biology and medicine to physics and engineering.

This only and up-to-date monograph on this versatile method covers its use in a range of applications spanning the fields of physics, materials science, electrical engineering, medicine, and research and industry. Following an introduction, the highly experienced author goes on to investigate acoustic field structure, output signal formation in transmission raster acoustic microscopes and non-linear acoustic effects. Further chapters deal with the visco-elastic properties and microstructure of the model systems and composites used, as well as polymer composite materials and the microstructure and physical-mechanical properties of biological tissues. A handy reference for materials scientists, electrical engineers, radiologists, laboratory medics, test engineers, physicists, and graduate students.

Previous edition won First Prize in the Gastroenterology category of the 2008 BMA Medical Book Competition High-resolution endoscopy and narrowband imaging have revolutionized the field. Edited by a gastroenterologist with a reputation for delivering outstanding material, this new edition of an award-winning atlas provides you with an outstanding collection of images, videos, and expert diagnostic guidance to enhance your decision making. To accelerate your learning, Dr. Cohen offers more than 2000 endoscopic images, emphasizing conditions for which NBI is particularly useful – such as finding dysplasia in Barrett's mucosa, and diagnosing adenomatous colon polyps – and providing exceptional preparation for the future of endoscopy practice, with a broad new look at normal and abnormal findings throughout the GI tract. The book is divided into three main parts: The basics of NBI Clinical applications of NBI Atlas of 1600 color images, broken into sections on the pharynx and esophagus, stomach, small intestine, and colon, including correlating histopathology and multiple examples of key pathologies The accompanying website features more than 85 video clips containing over 3 hours of annotated video, to give you a complete sense of how HRE and NBI work and look in real time, including during therapeutic procedures. All of the over 1000 new images appear in much brighter color, reflecting the advance in scope technology since the first edition. New chapters have been added to present the data supporting increased use of NBI in optical diagnosis and in the context of therapeutic procedures. For the first time, brilliant images of the bile duct and pancreas are included as the imaging revolution has expanded to reach these new locations. This spectacular new imaging modality promises to enhance endoscopic decision making in real time, facilitate therapeutic maneuvers, make tissue sampling more precise, and make resection of mucosal neoplasia more complete. Expertly guiding you through the latest advances, this book facilitates your mastery of the field, and provides an up-to-date reference for gastroenterologists and endoscopists to improve their practice.

Over the last decade, advances in science and technology have profoundly changed the face of light microscopy. Research scientists need to learn new skills in order to use a modern research microscope–skills such as how to align microscope optics and perform image processing. Fundamentals of Light Microscopy and Electronic Imaging explores the basics of microscope design and use. The comprehensive material discusses the optical principles involved in diffraction and image formation in the light microscope, the basic modes of light microscopy, the components of modern electronic imaging systems, and the image processing operations necessary to acquire and prepare an image. Written in a practical, accessible style, Fundamentals of Light Microscopy and Electronic Imaging reviews such topics as: Illuminators, filters, and isolation of specific wavelengths Phase contrast and differential interference contrast Properties of polarized light and polarization microscopy Fluorescence and confocal laser scanning microscopy Digital CCD microscopy and image processing Each chapter includes practical demonstrations and exercises along with a discussion of the relevant material. In addition, a thorough glossary assists with complex terminology and an appendix contains lists of materials, procedures for specimen preparation, and answers to questions. An essential resource for both, experienced and novice microscopists.

Fundamentals of Light Microscopy and Electronic Imaging, Second Edition provides a coherent introduction to the principles and applications of the integrated optical microscope system, covering both theoretical and practical considerations. It expands and updates discussions of multi-spectral imaging, intensified digital cameras, signal colocalization, and uses of objectives, and offers guidance in the selection of microscopes and electronic cameras, as well as appropriate auxiliary optical systems and fluorescent tags. The book is divided into three sections covering optical principles in diffraction and image formation, basic modes of light microscopy, and components of modern electronic imaging systems and image processing operations. Each chapter introduces relevant theory, followed by descriptions of instrument alignment and image interpretation. This revision includes new chapters on live cell imaging, measurement of protein dynamics, deconvolution microscopy, and interference microscopy. PowerPoint slides of the figures as well as other supplementary materials for instructors are available at a companion website: www.wiley.com/go/murphy/lightmicroscopy

This book provides students, teachers, researchers and clinicians with a strong and established source of information on advanced optical technologies that show real promise of being translated to clinical use.

This volume is a collection of twenty-two cutting edge research papers from the symposia on Nano-Biotechnology and Ceramics in Biomedical Applications and Advances in Biomineralized Ceramics, Bioceramics, and Bioinspired Designs, which were presented at the 8th Pacific Rim Conference on Ceramics and Glass Technology (PACRIM-8). The symposia was focused on several key areas, including novel synthesis techniques, bioglasses and glass-ceramics, calcium phosphates for bone tissue applications, and oxide ceramic implant applications. These papers cut across disciplines – ceramic science and technology, bioengineering and nanoscience – showing that a new, exciting field has emerged in the ceramics community.

This book contains 26 papers from the Magnetoelectric Multiferroic Thin Films and Multilayers; Dielectric Ceramic Materials and Electronic Devices; Recent Developments in High-Temperature Superconductivity; and Multifunctional Oxides symposia held during the 2010 Materials Science and Technology (MS&T'10) meeting, October 17-21, 2010, Houston, Texas. Topics include: Properties; Structures; Synthesis; Characterization; Device Applications; Multiferroics and Magnetoelectrics; YBCO Pinning Methods and Properties; YBCO Processing and Reliability Related Issues; New Superconductors and MgB2.

The use of ceramics in biological environments and biomedical applications is of increasing importance, as is the understanding of how biology works with minerals to develop strong materials. Specific information about biomimetics, and processing, performance and interactions of materials for biomedical applications is presented in this collection.

A collection of articles from the Advances in Biomedical and Biomimetic Materials symposium give insight into advances in biomedical and biomimetic materials. These selected articles cover such topics as scaffolds for tissue engineering, bioceramics, biomimetic materials, nanoparticles for medical diagnosis and treatment, and novel materials for drug delivery and biosensing.