Practical Flow Cytometry

The analysis of blood, bone marrow and tissue fluid specimens requires a multi-faceted approach with the integration of scientific data from a number of disciplines. No single discipline can operate in isolation or errors will occur. Flow cytometry is in a privileged position in that it can provide rapid analysis of specimens and it is often the first definitive investigation to produce results and help formulate a working diagnosis. This companion text to Practical Flow Cytometry in Haematology Diagnosis contains 100 worked examples drawn from real clinical cases presenting to the authors’ institution. Cases are illustrated with peripheral blood and bone marrow cytology, tissue pathology and cytogenetic and molecular data, which are integrated to generate, where appropriate, a diagnosis based on the WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues. The spectrum of clinical cases includes adult and paediatric patients, and both neoplastic and reactive disorders. The cases appear in no particular order to challenge the reader to make their own diagnosis. The reader will review May−Gr?nwald−Giemsa (MGG)-stained films of peripheral blood and bone marrow aspirates presented alongside flow cytometric data and haematoxylin and eosin (H&E)-stained bone marrow and other tissue biopsy sections. Immunohistochemistry is used to further clarify the tissue lineage and cell differentiation. Cytogenetic studies using metaphase preparations are used to identify translocations and chromosome gains and losses whilst interphase fluorescence in situ hybridisation (FISH) studies and polymerase chain reaction (PCR) are used to identify gene fusions, gene rearrangements and deletions. Each case concludes with a discussion of the features that are important to making a diagnosis. The cases are also listed according to disease classification in the appendix so that the text can also be used as a reference. Practical Flow Cytometry in Haematology: 100 Worked Examples: Provides a practical, example-based resource for flow cytometry Demonstrates how flow cytometry results should be interpreted and applied to optimize patient care Includes both malignant and benign conditions Can be used in conjunction with Practical Flow Cytometry in Haematology Diagnosis, by the same author team (ISBN 9780470671207) Practical Flow Cytometry in Haematology: 100 Worked Examples is ideal for practicing haematologists and histopathologists with an interest in haematopathology, but particularly directed at trainee haematologists and scientists preparing for FRCPath and related examinations.

The accurate diagnosis of haematologic malignancies is a complex and challenging task. It routinely involves morphologic, molecular, cytogenetic and flow cytometric expertise. To determine what treatment protocol will be followed, it is vital to integrate, interpret and report these results accurately. Flow cytometry is key in this diagnostic pathway. This book guides the reader as to how flow cytometry results should be interpreted and applied to optimize patient care. At the core of this text is an appreciation of clinical, morphological and immunophenotypic correlation and the importance of constant liaison and discussion between the medical and scientific teams. The authors present a logical and practical approach to the diagnosis of blood disorders (both neoplastic and reactive) and evaluate the diagnostic applications of flow cytometry. Practical Flow Cytometry in Haematology Diagnosis provides: • A clinical reference source on all aspects of flow cytometry, covering both malignant and benign conditions • Carefully chosen real-life cases in each chapter, complemented by high quality morphological images • Help in making a diagnosis, together with an understanding of the limitations of the technique and the potential pitfalls All those who instigate, perform, interpret or act upon flow cytometry patient material will find this book an invaluable guide.

Flow Cytometry of Hematological Malignancies contains an array of graphical outputs produced by the technique in the study of the most (and the least) common diseases. The images included allow you to compare your own results with a third party reference pattern. There is a detailed description of the main leukocyte antigens, together with a description of their distribution amongst normal and abnormal blood cells. The book also provides a comprehensive description of the phenotype of every neoplastic blood disease recorded in the WHO classification system, including all the instructions needed to recognise and classify even the least common entity. Designed to be practical, the book is perfect for quick consultation and is divided into two main sections. Section I deals with the direct object of immunophenotyping, and Section II deals with the ultimate target of the analysis. More than 50 antigens are covered and every antigen is dealt with in three main parts: general features, cytometric features and practical hints. This authoritative and state-of-the-art reference will be invaluable for clinicians directly involved in the diagnosis and analysis of hematological diseases, including hematologists, hematopathologists, oncologists, pathologists and technicians working in diagnostic laboratories.

Flow Cytometry of Hematological Malignancies contains an array of graphical outputs produced by the technique in the study of the most (and the least) common diseases. The images included allow you to compare your own results with a third party reference pattern. There is a detailed description of the main leukocyte antigens, together with a description of their distribution amongst normal and abnormal blood cells. The book also provides a comprehensive description of the phenotype of every neoplastic blood disease recorded in the WHO classification system, including all the instructions needed to recognise and classify even the least common entity. Designed to be practical, the book is perfect for quick consultation and is divided into two main sections. Section I deals with the direct object of immunophenotyping, and Section II deals with the ultimate target of the analysis. More than 50 antigens are covered and every antigen is dealt with in three main parts: general features, cytometric features and practical hints. This authoritative and state-of-the-art reference will be invaluable for clinicians directly involved in the diagnosis and analysis of hematological diseases, including hematologists, hematopathologists, oncologists, pathologists and technicians working in diagnostic laboratories.

Targeted at beginners as well as experienced users, this handy reference explains the benefits and uses of flow cytometery in the study of plants and their genomes. Following a brief introduction that highlights general considerations when analyzing plant cells by flow cytometric methods, the book goes on to discuss examples of application in plant genetics, genomic analysis, cell cycle analysis, marine organism analysis and breeding studies. With its list of general reading and a glossary of terms, this first reference on FCM in plants fills a real gap by providing first-hand practical hints for the growing community of plant geneticists.

This book covers the unique application of flow cytometry in drug discovery and development. The first section includes two introductory chapters, one on flow cytometry and one on biomarkers, as well as a chapter on recent advances in flow cytometry. The second section focuses on the unique challenges and added benefits associated with the use of flow cytometry in the drug development process. The third section contains a single chapter presenting an in depth discussion of validation considerations and regulatory compliance issues associated with drug development.

A much-needed primer on the use of laser flow cytometry for stem cell analysis Laser flow cytometry is a powerful tool for rapid analysis of cells for marker expression, cell cycle position, proliferation, and apoptosis. However, no resources specifically address the use of this methodology for the study of stem cells; this is especially important as stem cell analysis involves specialized methods and staining procedures based on specific characteristics such as marker expression, cell size, drug transport, and efflux of the stem cells. Now, this book reviews these procedures, discusses the science behind them, and provides real-world examples to illustrate the usefulness of the methods. It brings together world-class experts in pathology, biophysics, immunology, and stem cell research, who draw upon their extensive experience with the methods and show examples of good data to help guide researchers in the right direction. Chapter coverage includes: Stem cell analysis and sorting using side population Flow cytometry in the study of proliferation and apoptosis Stem cell biology and application Identification and isolation of very small embryonic-like stem cells from murine and human specimens Hematopoietic stem cells—issues in enumeration Human embryonic stem cells: long-term culture and cardiovascular differentiation Limbal stem cells and corneal regeneration Flow cytometric sorting of spermatogonial stem cells Breast cancer stem cells Stem cell marker expression in cells from body cavity fluids This book is an essential resource for all graduate students, practitioners in developing countries, libraries and book repositories of universities and research institutions, and individual researchers. It is also of interest to laboratories engaged in stem cell research and use of stem cells for tissue regeneration, and to any organization dealing in stem cell and tissue regeneration research.

Линейная щелевая решетка FLOW (Флоу) Model-X изготовлена из алюминия высокого качества и покрыта полимерной краской, обеспечивающей защиту от коррозии. Оборудование позволит создать комфортный приток воздуха и отличается продуманной конструкцией. Данная модель предназначена для скрытого потолочного монтажа. Возможны различные варианты покрытий, включая порошковую покраску в палитру RAL, покрытие цветными металлами, декоративную отделку шпоном ценных пород деревьев и нанесение гравировки или печати изображений на видимую часть изделия. Цена указана за 1 п.м. Особенности и преимущества: Угловые элементы могут быть изготовлены под любым углом, что позволяет использовать его в помещениях различной формы. Возможность соединения нескольких решеток в одну систему вентиляции, обеспечивая большую площадь покрытия. Изделие может быть установлено в различные типы поверхностей, такие как гипсокартон (ГКЛ), керамическая плитка, МДФ, штукатурка и другие материалы. Отражатель обеспечивает визуальную эстетику и скрывает элементы вентиляции. Конструкция обеспечивает равномерное распределение воздушного потока. Большой выбор покрытий позволяет подобрать решетку под любой интерьер. Добавление в порошковую краску антибактериальных и антистатических свойств. Изготовление камеры статического давления с учетом любых потребностей. Уникальная форма профиля гарантирует идеальную геометрию решетки. Разработка и испытания диффузоров проводится на базе МГТУ им. Н.Э. Баумана, все технические характеристики решеток подтверждены в ходе реальных испытаний. Щелевые решетки от компании FLOW применяются для подачи воздуха в помещение и представлены моделями различных конфигураций. Оборудование может быть изготовлено специально по размерам заказчика. Представленные решетки подходят для любых потолочных покрытий и отличаются высоким качеством используемых материалов.

Линейная щелевая решетка FLOW (Флоу) Model-T отличается надежностью и долгим сроком службы, так как изготавливается из качественного алюминиевого сплава. На металл наносится полимерное покрытие, которое обеспечивает антикоррозийную защиту. Устройство предполагает скрытый монтаж и подходит ко всем типам потолочных покрытий. Возможны различные варианты покрытий, включая порошковую покраску в палитру RAL, покрытие цветными металлами, декоративную отделку шпоном ценных пород деревьев и нанесение гравировки или печати изображений на видимую часть изделия. Цена указана за 1 п.м. Особенности и преимущества: Угловые элементы могут быть изготовлены под любым углом, что позволяет использовать его в помещениях различной формы. Возможность соединения нескольких решеток в одну систему вентиляции, обеспечивая большую площадь покрытия. Изделие может быть установлено в различные типы поверхностей, такие как гипсокартон (ГКЛ), керамическая плитка, МДФ, штукатурка и другие материалы. Отражатель обеспечивает визуальную эстетику и скрывает элементы вентиляции. Конструкция обеспечивает равномерное распределение воздушного потока. Большой выбор покрытий позволяет подобрать решетку под любой интерьер. Добавление в порошковую краску антибактериальных и антистатических свойств. Изготовление камеры статического давления с учетом любых потребностей. Уникальная форма профиля гарантирует идеальную геометрию решетки. Разработка и испытания диффузоров проводится на базе МГТУ им. Н.Э. Баумана, все технические характеристики решеток подтверждены в ходе реальных испытаний. Щелевые решетки от компании FLOW применяются для подачи воздуха в помещение и представлены моделями различных конфигураций. Оборудование может быть изготовлено специально по размерам заказчика. Представленные решетки подходят для любых потолочных покрытий и отличаются высоким качеством используемых материалов.

Practical Multiscaling covers fundamental modelling techniques aimed at bridging diverse temporal and spatial scales ranging from the atomic level to a full-scale product level. It focuses on practical multiscale methods that account for fine-scale (material) details but do not require their precise resolution. The text material evolved from over 20 years of teaching experience at Rensselaer and Columbia University, as well as from practical experience gained in the application of multiscale software. This book comprehensively covers theory and implementation, providing a detailed exposition of the state-of-the-art multiscale theories and their insertion into conventional (single-scale) finite element code architecture. The robustness and design aspects of multiscale methods are also emphasised, which is accomplished via four building blocks: upscaling of information, systematic reduction of information, characterization of information utilizing experimental data, and material optimization. To ensure the reader gains hands-on experience, a companion website hosting a lite version of the multiscale design software (MDS-Lite) is available. Key features: Combines fundamental theory and practical methods of multiscale modelling Covers the state-of-the-art multiscale theories and examines their practical usability in design Covers applications of multiscale methods Accompanied by a continuously updated website hosting the multiscale design software Illustrated with colour images Practical Multiscaling is an ideal textbook for graduate students studying multiscale science and engineering. It is also a must-have reference for government laboratories, researchers and practitioners in civil, aerospace, pharmaceutical, electronics, and automotive industries, and commercial software vendors.