Practical Methods for Biocatalysis and Biotransformations

Biocatalysts are increasingly used by chemists engaged in fine chemical synthesis within both industry and academia. Today, there exists a huge choice of high-tech enzymes and whole cell biocatalysts, which add enormously to the repertoire of synthetic possibilities. Practical Methods for Biocatalysis and Biotransformations 3 will be a companion book to Practical Methods for Biocatalysis and Biotransformations (2009) and Practical Methods for Biocatalysis and Biotransformations 2 (2012). Following the successful format of the two volumes, it will be a “how-to” guide focusing on commercially available enzymes and strains of microorganisms that are readily obtained from culture collections. The source of starting materials and reagents, hints, tips and safety advice (where appropriate) will be given to ensure, as far as possible, that the procedures are reproducible. Comparisons to alternative methodology will be given and relevant references to the primary literature will be cited. Contents include: Biotransformation Process Technology Industrial Biooxidation Hydrolase catalysed hydrolysis/synthesis Reduction Oxidation Halogenation Transferase catalysed glycosylation, methylation, etc C-C bond formation Tandem Biocatalytic Reactions Practical Methods for Biocatalysis and Biotransformations, Volume 3 is an essential collection of validated biocatalytic methods which will find a place on the bookshelves of synthetic organic chemists, pharmaceutical chemists, and process R&D chemists in industry and academia.

Biocatalysts are increasingly used by chemists engaged in fine chemical synthesis within both industry and academia. Today, there exists a huge choice of high-tech enzymes and whole cell biocatalysts, which add enormously to the repertoire of synthetic possibilities. Practical Methods for Biocatalysis and Biotransformations 2 is a «how-to» guide that focuses on the practical applications of enzymes and strains of microorganisms that are readily obtained or derived from culture collections. The sources of starting materials and reagents, hints, tips and safety advice (where appropriate) are given to ensure, as far as possible, that the procedures are reproducible. Comparisons to alternative methodology are given and relevant references to the primary literature are cited. This second volume – which can be used on its own or in combination with the first volume – concentrates on new applications and new enzyme families reported since the first volume. Contents include: introduction to recent developments and future needs in biocatalysts and synthetic biology in industry reductive amination enoate reductases for reduction of electron deficient alkenes industrial carbonyl reduction regio- and stereo- selective hydroxylation oxidation of alcohols selective oxidation industrial hydrolases and related enzymes transferases for alkylation, glycosylation and phosphorylation C-C bond formation and decarboxylation halogenation/dehalogenation/heteroatom oxidation tandem and sequential multi-enzymatic syntheses Practical Methods for Biocatalysis and Biotransformations 2 is an essential collection of biocatalytic methods for chemical synthesis which will find a place on the bookshelves of synthetic organic chemists, pharmaceutical chemists, and process R&D chemists in industry and academia.

The development of new asymmetric catalytic methods is of fundamental importance to industrial synthetic chemistry. The demand for optically pure synthetic intermediates and the drive to adopt greener methods of synthesis have stimulated a growing interest in biocatalysis as a selective and environmentally benign synthetic technique. Practical Biotransformations: A Beginner's Guide provides an introduction to microbes and enzymes and demonstrates their practical applications in synthetic organic chemistry. Designed as a laboratory manual, this user-friendly guide discusses standard laboratory techniques, with appropriate advice on aspects of microbial practice and associated safety. Topics covered include: An introduction to equipment in a biotransformations laboratory An overview of biocatalyst sources Maintenance and growth of biocatalysts Example biotransformations using commercially available microbes and enzymes Basic gene cloning and the use of ‘designer’ biocatalysts This book will be a valuable resource for synthetic organic chemists with little or no experience of biochemistry or microbiology. It is the author’s hope that this text will inspire readers to consider biocatalytic methods as real alternatives to traditional synthetic solutions.

Large surveys are becoming increasingly available for public use, and researchers are often faced with the need to analyse complex survey data to address key scientific issues. For proper analysis it is also important to be aware of the different aspects of the design of complex surveys. Practical Methods for Design and Analysis of Complex Surveys features intermediate and advanced statistical techniques for use in designing and analysing complex surveys. This extensively updated edition features much new material, and detailed practical exercises with links to a Web site, helping instructors and enabling use for distance learning. * Provides a comprehensive introduction to sampling and estimation in descriptive surveys, including design effect statistic and use of auxiliary data. * Includes detailed coverage of complex survey analysis, including design-based ANOVA and logistic regression with GEE estimation. * Contains much new material, including handling of non-sampling errors, and model-assisted estimation for domains. * Features detailed real-li fe case studies, such as multilevel modeling in a multinational educational survey. * Supported by a Web site containing software codes, real data sets, computerized exercises with solutions, and online training materials. Practical Methods for Design and Analysis of Complex Surveys provides a useful practical resource for researchers and practitioners working in the planning, implementation or analysis of complex surveys and opinion polls, including business, educational, health, social, and socio-economic surveys and official statistics. In addition, the book is well suited for use on intermediate and advanced courses in survey sampling.

An up-to-date overview of diverse findings and accomplishments in biocatalysis and bioenergy With the high price of petroleum and researchers worldwide seeking new means of producing energy, this comprehensive book on biocatalysis for bioenergy and biofuel applications is very timely. It combines information on state-of-the-art advances and in-depth reviews of the latest achievements in biocatalysis and bioenergy, emphasizing biodiesel, bioethanol, and industrial products. The advantages of biocatalysis include high specificity, efficiency, energy conservation, and pollution reduction. Biocatalysis and Bioenergy details advances in the field, with: * Three primary sections, covering biodiesel research, bioethanol, and industrial products * Information on enzyme catalysis, biotransformation, bioconversion, fermentation, genetic engineering, and product recovery * * Contributions from leading experts worldwide who share their research and findings The prospect of using biocatalysis for the production of energy has great potential due to its cost-effectiveness, the fact that it does not require a limited resource such as oil, and its potential universality of application and use globally. This is the definitive reference for biochemists and biochemical engineers, bioprocess and bioenergy scientists, physical and oil chemists (oleochemists), microbiologists, industrial microbiologists, molecular biologists, metabolic engineers working in biocatalysis, bioethanol, and biodiesel fuels, DOE scientists working on renewable energy, and other professionals in related fields.

An expert overview of new technologies guiding the construction of a sustainable society This compendium of important insights from sixty distinguished international scholars looks at the significant advances in progressive environmental technology—especially the molecular engineering used on plants, animals, and microorganisms—as the game changer in the high-stakes race to reverse earth-damaging practices. Biocatalysis and Biomolecular Engineering covers subject matter on the latest developments in eco-friendly and energy-saving manufacturing processes with the emphasis on agricultural technology and bio-based products. Focusing its study on remedies that show promise in curing food and energy ills, this book examines groundbreaking work in various fields, such as nutraceuticals, genetic engineering of agricultural products, and bioenergy. Biocatalysis and Biomolecular Engineering: Can be used as a reference by teachers, graduate students, and industrial scientists who conduct research in bioscience and biotechnology Serves as the first book to bring together fundamentals and leading-edge technologies for the development of bio-based industrial products through biocatalysis; for example, it discusses the preparation of biofunctional micro- and nanoparticles Contains chapters by international experts from academia, industry, and government research institutes Biocatalysis and Biomolecular Engineering builds a cohesive, well thought out case for nurturing new discoveries in eco-technology by inviting critical discussion on devising viable solutions to sustaining the future wellness of humankind.

This book describes recent progress in enzyme-driven green syntheses of industrially important molecules. The first three introductory chapters overview recent technological advances in enzymes and cell-based transformations, and green chemistry metrics for synthetic efficiency. The remaining chapters are directed to case studies in biotechnological production of pharmaceuticals (small molecules, natural products and biologics), flavors, fragrance and cosmetics, fine chemicals, value-added chemicals from glucose and biomass, and polymeric materials. The book is aimed to facilitate the industrial applications of this powerful and emerging green technology, and catalyze the advancement of the technology itself.

Biocatalysis is rapidly evolving into a key technology for the discovery and production of chemicals, especially in the pharmaceutical industry, where high yielding chemo-, regio-, and enantioselective reactions are critical. Taking the latest breakthroughs in genomics and proteomics into consideration, Biocatalysis for the Pharmaceutical Industry concisely yet comprehensively discusses the modern application of biocatalysis to drug discovery, development, and manufacturing. Written by a team of leading experts, the book offers deep insight into this cutting edge field. Covers a wide range of topics in a systematic manner with an emphasis on industrial applications Provides a thorough introduction to the latest biocatalysts, modern expression hosts, state-of-the-art directed evolution, high throughput screening, and bioprocess engineering Addresses frontier subjects such as emerging enzymes, metabolite profiling, combinatorial biosynthesis, metabolic engineering, and autonomous enzymes for the synthesis and development of chiral molecules, drug metabolites, and semi-synthetic medicinal compounds and natural product analogs Highlights the impact of biocatalysis on green chemistry Contains numerous graphics to illustrate concepts and techniques Biocatalysis for the Pharmaceutical Industry is an essential resource for scientists, engineers, and R&D policy makers in the fine chemical, pharmaceutical, and biotech industries. It is also an invaluable tool for academic researchers and advanced students of organic and materials synthesis, chemical biology, and medicinal chemistry.

Before the 1970s, most information concerning the conservation and restoration of paintings, wood, and archaeological artefacts were focused on the history of the artefacts, previous attempts of conservation, and the future use of these artefacts. The technical methods of how the restoration and conservation were made were dealt with only very briefly. Today, sophisticated methods of scientific analysis such as DNA are common place, and this encourages conservators and scientists to work together to work out the development of new methods for analysis and conservation of artefacts. This book focuses on the chemicals used for conservation and restoration of various artefacts in artwork and archaeology, as well as special applications of these materials. Also the methods used, both methods for cleaning, conservation and restoration, as well as methods for the analysis of the state of the respective artefacts. Topics include oil paintings, paper conservation, textiles and dyes for them, archaeological wood, fossils, stones, metals and metallic coins, and glasses, including church windows.

Fully describes optimization methods that are currently most valuable in solving real-life problems. Since optimization has applications in almost every branch of science and technology, the text emphasizes their practical aspects in conjunction with the heuristics useful in making them perform more reliably and efficiently. To this end, it presents comparative numerical studies to give readers a feel for possibile applications and to illustrate the problems in assessing evidence. Also provides theoretical background which provides insights into how methods are derived. This edition offers revised coverage of basic theory and standard techniques, with updated discussions of line search methods, Newton and quasi-Newton methods, and conjugate direction methods, as well as a comprehensive treatment of restricted step or trust region methods not commonly found in the literature. Also includes recent developments in hybrid methods for nonlinear least squares; an extended discussion of linear programming, with new methods for stable updating of LU factors; and a completely new section on network programming. Chapters include computer subroutines, worked examples, and study questions.