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This unique book on super-resolution microscopy techniques presents comparative, in-depth analyses of the strengths and weaknesses of the individual approaches. It was written for non-experts who need to understand the principles of super-resolution or who wish to use recently commercialized instruments as well as for professionals who plan to realize novel microscopic devices. Explaining the practical requirements in terms of hardware, software and sample preparation, the book offers a wealth of hands-on tips and practical tricks to get a setup running, provides invaluable help and support for successful data acquisition and specific advice in the context of data analysis and visualization. Furthermore, it addresses a wide array of transdisciplinary fields of applications. The author begins by outlining the joint efforts that have led to achieving super-resolution microscopy combining advances in single-molecule photo-physics, fluorophore design and fluorescent labeling, instrument design and software development. The following chapters depict and compare current main standard techniques such as structured illumination microscopy, single-molecule localization, stimulated emission depletion microscopy and multi-scale imaging including light-sheet and expansion microscopy. For each individual approach the experimental setups are introduced, the imaging protocols are provided and the various applications illustrated. The book concludes with a discussion of future challenges addressing issues of routine applications and further commercialization of the available methods. Guiding users in how to make choices for the design of their own experiments from scratch to promising application, this one-stop resource is intended for researchers in the applied sciences, from chemistry to biology and medicine to physics and engineering.

Introduces the applications of repeated measures design processes with the popular IBM® SPSS® software Repeated Measures Design for Empirical Researchers presents comprehensive coverage of the formation of research questions and the analysis of repeated measures using IBM SPSS and also includes the solutions necessary for understanding situations where the designs can be used. In addition to explaining the computation involved in each design, the book presents a unique discussion on how to conceptualize research problems as well as identify appropriate repeated measures designs for research purposes. Featuring practical examples from a multitude of domains including psychology, the social sciences, management, and sports science, the book helps readers better understand the associated theories and methodologies of repeated measures design processes. The book covers various fundamental concepts involved in the design of experiments, basic statistical designs, computational details, differentiating independent and repeated measures designs, and testing assumptions. Along with an introduction to IBM SPSS software, Repeated Measures Design for Empirical Researchers includes: A discussion of the popular repeated measures designs frequently used by researchers, such as one-way repeated measures ANOVA, two-way repeated measures design, two-way mixed design, and mixed design with two-way MANOVA Coverage of sample size determination for the successful implementation of designing and analyzing a repeated measures study A step-by-step guide to analyzing the data obtained with real-world examples throughout to illustrate the underlying advantages and assumptions A companion website with supplementary IBM SPSS data sets and programming solutions as well as additional case studies Repeated Measures Design for Empirical Researchers is a useful textbook for graduate- and PhD-level students majoring in biostatistics, the social sciences, psychology, medicine, management, sports, physical education, and health. The book is also an excellent reference for professionals interested in experimental designs and statistical sciences as well as statistical consultants and practitioners from other fields including biological, medical, agricultural, and horticultural sciences. J. P. Verma, PhD, is Professor of Statistics and Director of the Center for Advanced Studies at Lakshmibai National Institute of Physical Education, India. Professor Verma is an active researcher in sports modeling and data analysis and has conducted many workshops on research methodology, research designs, multivariate analysis, statistical modeling, and data analysis for students of management, physical education, social science, and economics. He is the author of Statistics for Exercise Science and Health with Microsoft® Office Excel®, also published by Wiley.

Ecosystem Dynamics focuses on long-term terrestrial ecosystems and their changing relationships with human societies. The unique aspect of this text is the long-time scale under consideration as data and insights from the last 10,000 years are used to place present-day ecosystem status into a temporal perspective and to test models that generate forecasts of future conditions. Descriptions and assessments of some of the current modelling tools that are used, along with their uncertainties and assumptions, are an important feature of this book. An overarching theme explores the dynamic interactions between human societies and ecosystem functioning and services. This book is authoritative but accessible and provides a useful background for all students, practitioners, and researchers interested in the subject.

Diagnostic Electron Microscopy: A Practical Guide to Interpretation and Technique summarises the current interpretational applications of TEM in diagnostic pathology. This concise and accessible volume provides a working guide to the main, or most useful, applications of the technique including practical topics of concern to laboratory scientists, brief guides to traditional tissue and microbiological preparation techniques, microwave processing, digital imaging and measurement uncertainty. The text features both a screening and interpretational guide for TEM diagnostic applications and current TEM diagnostic tissue preparation methods pertinent to all clinical electron microscope units worldwide. Containing high-quality representative images, this up-to-date text includes detailed information on the most important diagnostic applications of transmission electron microscopy as well as instructions for specific tissues and current basic preparative techniques. The book is relevant to trainee pathologists and practising pathologists who are expected to understand and evaluate/screen tissues by TEM. In addition, technical and scientific staff involved in tissue preparation and diagnostic tissue evaluation/screening by TEM will find this text useful.

A comprehensive guide to the art and science of bioimaging data acquisition, processing and analysis Standard and Super-Resolution Bioimaging Data Analysis gets newcomers to bioimage data analysis quickly up to speed on the mathematics, statistics, computing hardware and acquisition technologies required to correctly process and document data. The past quarter century has seen remarkable progress in the field of light microscopy for biomedical science, with new imaging technologies coming on the market at an almost annual basis. Most of the data generated by these systems is image-based, and there is a significant increase in the content and throughput of these imaging systems. This, in turn, has resulted in a shift in the literature on biomedical research from descriptive to highly-quantitative. Standard and Super-Resolution Bioimaging Data Analysis satisfies the demand among students and research scientists for introductory guides to the tools for parsing and processing image data. Extremely well illustrated and including numerous examples, it clearly and accessibly explains what image data is and how to process and document it, as well as the current resources and standards in the field. A comprehensive guide to the tools for parsing and processing image data and the resources and industry standards for the biological and biomedical sciences Takes a practical approach to image analysis to assist scientists in ensuring scientific data are robust and reliable Covers fundamental principles in such a way as to give beginners a sound scientific base upon which to build Ideally suited for advanced students having only limited knowledge of the mathematics, statistics and computing required for image data analysis An entry-level text written for students and practitioners in the bioscience community, Standard and Super-Resolution Bioimaging Data Analysis de-mythologises the vast array of image analysis modalities which have come online over the past decade while schooling beginners in bioimaging principles, mathematics, technologies and standards.