RNA and DNA Editing

Discover the science of biocomputing with this comprehensive and forward-looking new resource DNA- and RNA-Based Computing Systems delivers an authoritative overview of DNA- and RNA-based biocomputing systems that touches on cutting-edge advancements in computer science, biotechnology, nanotechnology, and materials science. Accomplished researcher, academic, and author Evgeny Katz offers readers an examination of the intersection of computational, chemical, materials, and engineering aspects of biomolecular information processing. A perfect companion to the recently published Enzyme-Based Computing by the same editor, the book is an authoritative reference for those who hope to better understand DNA- and RNA-based logic gates, multi-component logic networks, combinatorial calculators, and related computational systems that have recently been developed for use in biocomputing devices. DNA- and RNA-Based Computing Systems summarizes the latest research efforts in this rapidly evolving field and points to possible future research foci. Along with an examination of potential applications in biosensing and bioactuation, particularly in the field of biomedicine, the book also includes topics like: A thorough introduction to the fields of DNA and RNA computing, including DNA/enzyme circuits A description of DNA logic gates, switches and circuits, and how to program them An introduction to photonic logic using DNA and RNA The development and applications of DNA computing for use in databases and robotics Perfect for biochemists, biotechnologists, materials scientists, and bioengineers, DNA- and RNA-Based Computing Systems also belongs on the bookshelves of computer technologists and electrical engineers who seek to improve their understanding of biomolecular information processing. Senior undergraduate students and graduate students in biochemistry, materials science, and computer science will also benefit from this book.

A practical, one-stop reference on the theory and applications of statistical data editing and imputation techniques Collected survey data are vulnerable to error. In particular, the data collection stage is a potential source of errors and missing values. As a result, the important role of statistical data editing, and the amount of resources involved, has motivated considerable research efforts to enhance the efficiency and effectiveness of this process. Handbook of Statistical Data Editing and Imputation equips readers with the essential statistical procedures for detecting and correcting inconsistencies and filling in missing values with estimates. The authors supply an easily accessible treatment of the existing methodology in this field, featuring an overview of common errors encountered in practice and techniques for resolving these issues. The book begins with an overview of methods and strategies for statistical data editing and imputation. Subsequent chapters provide detailed treatment of the central theoretical methods and modern applications, with topics of coverage including: Localization of errors in continuous data, with an outline of selective editing strategies, automatic editing for systematic and random errors, and other relevant state-of-the-art methods Extensions of automatic editing to categorical data and integer data The basic framework for imputation, with a breakdown of key methods and models and a comparison of imputation with the weighting approach to correct for missing values More advanced imputation methods, including imputation under edit restraints Throughout the book, the treatment of each topic is presented in a uniform fashion. Following an introduction, each chapter presents the key theories and formulas underlying the topic and then illustrates common applications. The discussion concludes with a summary of the main concepts and a real-world example that incorporates realistic data along with professional insight into common challenges and best practices. Handbook of Statistical Data Editing and Imputation is an essential reference for survey researchers working in the fields of business, economics, government, and the social sciences who gather, analyze, and draw results from data. It is also a suitable supplement for courses on survey methods at the upper-undergraduate and graduate levels.

Master the subjects of reversible computing and DNA computing with this expert volume   Reversible and DNA Computing  offers readers new ideas and technologies in the rapidly developing field of reversible computing. World-renowned researcher and author Hafiz Md. Hasan Babu shows readers the fundamental concepts and ideas necessary to understand reversible computing, including reversible circuits, reversible fault tolerant circuits, and reversible DNA circuits.  Reversible and DNA Computing  contains a practical approach to understanding energy-efficient DNA computing. In addition to explaining the foundations of reversible circuits, the book covers topics including:  Advanced logic design An introduction to the fundamentals of reversible computing Advanced reversible logic synthesis Reversible fault tolerance Fundamentals of DNA computing Reversible DNA logic synthesis DNA logic design This book is perfect for undergraduate and graduate students in the physical sciences and engineering, as well as those working in the field of quantum computing. It belongs on the bookshelves of anyone with even a passing interest in nanotechnology, energy-efficient computing, and DNA computing.

GENOME EDITING IN DRUG DISCOVERY A practical guide for researchers and professionals applying genome editing techniques to drug discovery In Genome Editing in Drug Discovery, a team of distinguished biologists delivers a comprehensive exploration of genome editing in the drug discovery process, with coverage of the technology’s history, current issues and techniques, and future perspectives and research directions. The book discusses techniques for disease modeling, target identification with CRISPR, safety studies, therapeutic editing, and intellectual property issues. The safety and efficacy of drugs and new target discovery, as well as next-generation therapeutics are also presented. Offering practical suggestions for practitioners and academicians involved in drug discovery, Genome Editing in Drug Discovery is a fulsome treatment of a technology that has become part of nearly every early step in the drug discovery pipeline. Selected contributions also include: A thorough introduction to the applications of CRISPRi and CRISPRa in drug discovery Comprehensive explorations of genome-editing applications in stem cell engineering and regenerative medicine Practical discussions of the safety aspects of genome editing with respect to immunogenicity and the specificity of CRISPR-Cas9 gene editing In-depth examinations of critical socio-economic and bioethical challenges in the CRISPR-Cas9 patent landscape Perfect for academic researchers and professionals in the biotech and pharmaceutical industries, Genome Editing in Drug Discovery will also earn a place in the libraries of medicinal chemists, biochemists, and molecular biologists.

Reverse genetics, the genetic manipulation of RNA viruses to create a wild-type or modified virus, has led to important advances in our understanding of viral gene function and interaction with host cells. Since many severe viral human and animal pathogens are RNA viruses, including those responsible for polio, measles, rotaviral diarrhoea and influenza infections, it is also an extremely powerful technique with important potential application for the prevention and control of a range of human and animal viral diseases. Reverse Genetics of RNA Viruses provides a comprehensive account of the very latest developments in reverse genetics of RNA viruses through a wide range of applications within each of the core virus groups including; positive sense, negative sense and double stranded RNA viruses. Written by a team of international experts in the field, it provides a unique insight into how the field has developed, what problems are being addressed now and where applications may lead in the future. It will prove invaluable to bioscience, medical and veterinary students, those starting research in this area as well as other researchers and teachers needing to update their knowledge of this fast-moving field. An authoritative, comprehensive overview of reverse genetics in RNA Viruses. Includes numerous examples of cutting- edge applications of reverse genetics within each of the RNA viral groups. Written by a team of international experts, including some of the leading researchers in the field.

Reverse genetics, the genetic manipulation of RNA viruses to create a wild-type or modified virus, has led to important advances in our understanding of viral gene function and interaction with host cells. Since many severe viral human and animal pathogens are RNA viruses, including those responsible for polio, measles, rotaviral diarrhoea and influenza infections, it is also an extremely powerful technique with important potential application for the prevention and control of a range of human and animal viral diseases. Reverse Genetics of RNA Viruses provides a comprehensive account of the very latest developments in reverse genetics of RNA viruses through a wide range of applications within each of the core virus groups including; positive sense, negative sense and double stranded RNA viruses. Written by a team of international experts in the field, it provides a unique insight into how the field has developed, what problems are being addressed now and where applications may lead in the future. It will prove invaluable to bioscience, medical and veterinary students, those starting research in this area as well as other researchers and teachers needing to update their knowledge of this fast-moving field. An authoritative, comprehensive overview of reverse genetics in RNA Viruses. Includes numerous examples of cutting- edge applications of reverse genetics within each of the RNA viral groups. Written by a team of international experts, including some of the leading researchers in the field.

A broad overview of the interaction of DNA with surfactants and polymers Due to the potential benefits of biotechnology, interest in the interaction between DNA and surfactants and polymers has become increasingly significant. Now, DNA Interactions with Polymers and Surfactants provides an extensive, up-to-date overview of the subject, giving readers a basis for understanding the factors leading to complexation between DNA and different cosolutes, including metal ions, polyelectrolytes, spermine, spermidine, surfactants and lipids, and proteins. Topical coverage includes: Polyelectrolytes, physico-chemical aspects and biological significance Solution behavior of nucleic acids Single DNA molecules: compaction and decompaction Interaction of DNA with surfactants and cationic polymers Interactions of histones with DNA DNA-DNA interactions The hydration of DNA-amphiphile complexes DNA-surfactant/lipid complexes at liquid interfaces DNA and DNA-surfactant complexes at solid surfaces The role of correlation forces for DNA-cosolute interactions Simulations of polyions Cross-linked DNA gels and gel particles DNA as an amphiphilic polymer Lipid-DNA interactions Covering both theoretical and practical aspects of the subject, DNA Interactions with Polymers and Surfactants is an ideal resource for chemists and biochemists working in gene and DNA delivery research in industry and academia, as well as for cell biologists, chemical engineers, molecular biologists, and development biologists in the pharmaceutical industry.

This book covers the emerging topic of DNA nanotechnology and DNA supramolecular chemistry in its broader sense. By taking DNA out of its biological role, this biomolecule has become a very versatile building block in materials chemistry, supramolecular chemistry and bio-nanotechnology. Many novel structures have been realized in the past decade, which are now being used to create molecular machines, drug delivery systems, diagnosis platforms or potential electronic devices. The book combines many aspects of DNA nanotechnology, including formation of functional structures based on covalent and non-covalent systems, DNA origami, DNA based switches, DNA machines, and alternative structures and templates. This broad coverage is very appealing since it combines both the synthesis of modified DNA as well as designer concepts to successfully plan and make DNA nanostructures. Contributing authors have provided first a general introduction for the non-specialist reader, followed by a more in-depth analysis and presentation of their topic. In this way the book is attractive and useful for both the non-specialist who would like to have an overview of the topic, as well as the specialist reader who requires more information and inspiration to foster their own research.

DNA technology is evolving rapidly, with new methods and a fast-growing vocabulary. This unique dictionary offers current, detailed and accessible information on DNA technology to lecturers, researchers and students throughout the biomedical and related sciences. The third edition is a major update, with over 3000 references from mainstream journals and data from the very latest research – going well beyond the remit of most science dictionaries. It provides clear explanations of terms, techniques, and tests, including commercial systems, with detailed coverage of many important procedures and methods, and includes essay-style entries on many major topics to assist newcomers to the field. It covers topics relevant to medicine (diagnosis, genetic disorders, gene therapy); veterinary science; biotechnology; biochemistry; pharmaceutical science/drug development; molecular biology; microbiology; epidemiology; genomics; environmental science; plant science/agriculture; taxonomy; and forensic science.