Sampling and Analysis of Indoor Microorganisms

An integrated approach to understanding the principles of sampling, chemical analysis, and instrumentation This unique reference focuses on the overall framework and why various methodologies are used in environmental sampling and analysis. An understanding of the underlying theories and principles empowers environmental professionals to select and adapt the proper sampling and analytical protocols for specific contaminants as well as for specific project applications. Covering both field sampling and laboratory analysis, Fundamentals of Environmental Sampling and Analysis includes: A review of the basic analytical and organic chemistry, statistics, hydrogeology, and environmental regulations relevant to sampling and analysis An overview of the fundamentals of environmental sampling design, sampling techniques, and quality assurance/quality control (QA/QC) essential to acquire quality environmental data A detailed discussion of: the theories of absorption spectroscopy for qualitative and quantitative environmental analysis; metal analysis using various atomic absorption and emission spectrometric methods; and the instrumental principles of common chromatographic and electrochemical methods An introduction to advanced analytical techniques, including various hyphenated mass spectrometries and nuclear magnetic resonance spectroscopy With real-life case studies that illustrate the principles plus problems and questions at the end of each chapter to solidify understanding, this is a practical, hands-on reference for practitioners and a great textbook for upper-level undergraduates and graduate students in environmental science and engineering.

A trusted classic on the key methods in population sampling—now in a modernized and expanded new edition Sampling of Populations, Fourth Edition continues to serve as an all-inclusive resource on the basic and most current practices in population sampling. Maintaining the clear and accessible style of the previous edition, this book outlines the essential statistical methodsfor survey design and analysis, while also exploring techniques that have developed over the past decade. The Fourth Edition successfully guides the reader through the basic concepts and procedures that accompany real-world sample surveys, such as sampling designs, problems of missing data, statistical analysis of multistage sampling data, and nonresponse and poststratification adjustment procedures. Rather than employ a heavily mathematical approach, the authors present illustrative examples that demonstrate the rationale behind common steps in the sampling process, from creating effective surveys to analyzing collected data. Along with established methods, modern topics are treated through the book's new features, which include: A new chapter on telephone sampling, with coverage of declining response rates, the creation of «do not call» lists, and the growing use of cellular phones A new chapter on sample weighting that focuses on adjustments to weight for nonresponse, frame deficiencies, and the effects of estimator instability An updated discussion of sample survey data analysis that includes analytic procedures for estimation and hypothesis testing A new section on Chromy's widely used method of taking probability proportional to size samples with minimum replacement of primary sampling units An expanded index with references on the latest research in the field All of the book's examples and exercises can be easily worked out using various software packages including SAS, STATA, and SUDAAN, and an extensive FTP site contains additional data sets. With its comprehensive presentation and wealth of relevant examples, Sampling of Populations, Fourth Edition is an ideal book for courses on survey sampling at the upper-undergraduate and graduate levels. It is also a valuable reference for practicing statisticians who would like to refresh their knowledge of sampling techniques.

This book helps evaluate the state of the art of rhizosphere microbial ecology and biotechnology. Experts in the field review methods and strategies applied to the detection, identification and monitoring of microorganisms in the rhizosphere. Major topics treated include: – construction of genetically marked rhizosphere bacteria – detection of marked wildtype and genetically modified organisms (GMOs) – identification of wildtype and GMOs by DNA probes and PCR amplification – rapid typing of non-modified and GMOs by PCR-based techniques – assessment of the role of gene transfer – EU regulations for the use and release of GMOs – biosafety results from field testing of GMOs In addition, technologies for the modifying gene expression and gene products for specific traits of agronomic interest in genetically engineered rhizosphere bacteria are covered.

A planar or two-dimensional (2D) mechanism is the combination of two or more machine elements that are designed to convey a force or motion across parallel planes. For any mechanical engineer, young or old, an understanding of planar mechanism design is fundamental. Mechanical components and complex machines, such as engines or robots, are often designed and conceptualised in 2D before being extended into 3D. Designed to encourage a clear understanding of the nature and design of planar mechanisms, this book favours a frank and straightforward approach to teaching the basics of planar mechanism design and the theory of machines with fully worked examples throughout. Key Features: Provides simple instruction in the design and analysis of planar mechanisms, enabling the student to easily navigate the text and find the desired material Covers topics of fundamental importance to mechanical engineering, from planar mechanism kinematics, 2D linkage analyses and 2D linkage design to the fundamentals of spur gears and cam design Shows numerous example solutions using EES (Engineering Equation Solver) and MATLAB software, with appendices dedicated to explaining the use of both computer tools Follows end-of-chapter problems with clearly detailed solutions

A multidisciplinary approach to understanding the fundamentals of mass spectrometry for bacterial analysis From chemotaxonomy to characterization of targeted proteins, Identification of Microorganisms by Mass Spectrometry provides an overview of both well-established and cutting-edge mass spectrometry techniques for identifying microorganisms. A vital tool for microbiologists, health professionals, and analytical chemists, the text is designed to help scientists select the most effective techniques for use in biomedical, biochemical, pharmaceutical, and bioterror defense applications. Since microbiological applications of mass spectrometry require a basic understanding of both microbiology and analytical chemistry, the editors have incorporated material from both disciplines so that readers from either field will come to understand the necessary principles of the other. Featuring contributions from some of the most recognized experts in both fields, this volume provides specific examples of fundamental methods as well as approaches developed in the last decade, including: * Metastable atom bombardment pyrolysis mass spectrometry * Matrix-assisted laser desorption/ionization mass spectrometry (MALDI) * MALDI time-of-flight mass spectrometry (MALDI-TOF MS) of intact bacteria * High-resolution Fourier transform mass spectrometry (FTMS) * Electrospray ionization (ESI) mass spectrometry Identification of Microorganisms by Mass Spectrometry represents the most comprehensive and up-to-date work on the topic currently available. It is liberally illustrated with figures and tables and covers every aspect of spectrometric identification of microorganisms, including experimental procedures, various means of sample preparation, data analysis, and interpretation of complex mass spectral data.

State-of-the-art coverage of modern computational methods for the analysis and design of beams Analysis and Design of Elastic Beams presents computer models and applications related to thin-walled beams such as those used in mechanical and aerospace designs, where thin, lightweight structures with high strength are needed. This book will enable readers to compute the cross-sectional properties of individual beams with arbitrary cross-sectional shapes, to apply a general-purpose computer analysis of a complete structure to determine the forces and moments in the individual members, and to use a unified approach for calculating the normal and shear stresses, as well as deflections, for those members' cross sections. In addition, this book augments a solid foundation in the basic structural design theory of beams by: * Providing coverage of thin-wall structure analysis and optimization techniques * Applying computer numerical methods to classical design methods * Developing computational solutions for cross-sectional properties and stresses using finite element analyses Including access to an associated Web site with software for the analysis and design of any cross-sectional shape, Analysis and Design of Elastic Beams: Computational Methods is an essential reference for mechanical, aerospace, and civil engineers and designers working in the automotive, ship, and aerospace industries in product and process design, machine design, structural design, and design optimization, as well as students and researchers in these areas.

Updated and expanded coverage of the latest trends and developments in fiber composite materials, processes, and applications Analysis and Performance of Fiber Composites, Fourth Edition features updated and expanded coverage of all technical aspects of fiber composites, including the latest trends and developments in materials, manufacturing processes, and materials applications, as well as the latest experimental characterization methods. Fiber reinforced composite materials have become a fundamental part of modern product manufacturing. Routinely used in such high-tech fields as electronics, automobiles, aircraft, and space vehicles, they are also essential to everyday staples of modern life, such as containers, piping, and appliances. Little wonder, when one considers their ease of fabrication, outstanding mechanical properties, design versatility, light weight, corrosion and impact resistance, and excellent fatigue strength. This Fourth Edition of the classic reference—the standard text for composite materials courses, worldwide—offers an unrivalled review of such an important class of engineering materials. Still the most comprehensive, up-to-date treatment of the mechanics, materials, performance, analysis, fabrication, and characterization of fiber composite materials available, Analysis and Performance of Fiber Composites, Fourth Edition features: Expanded coverage of materials and manufacturing, with additional information on materials, processes, and material applications Updated and expanded information on experimental characterization methods—including many industry specific tests Discussions of damage identification techniques using nondestructive evaluation (NDE) Coverage of the influence of moisture on performance of polymer matrix composites, stress corrosion of glass fibers and glass reinforced plastics, and damage due to low-velocity impact New end-of-chapter problems and exercises with solutions found on an accompanying website Computer analysis of laminates No other reference provides such exhaustive coverage of fiber composites with such clarity and depth. Analysis and Performance of Fiber Composites, Fourth Edition is, without a doubt, an indispensable resource for practicing engineers, as well as students of mechanics, mechanical engineering, and aerospace engineering.

Modeling and Analysis of Compositional Data presents a practical and comprehensive introduction to the analysis of compositional data along with numerous examples to illustrate both theory and application of each method. Based upon short courses delivered by the authors, it provides a complete and current compendium of fundamental to advanced methodologies along with exercises at the end of each chapter to improve understanding, as well as data and a solutions manual which is available on an accompanying website. Complementing Pawlowsky-Glahn’s earlier collective text that provides an overview of the state-of-the-art in this field, Modeling and Analysis of Compositional Data fills a gap in the literature for a much-needed manual for teaching, self learning or consulting.

A comprehensive overview of fluid dynamic models and experimental results that can help solve problems in centrifugal compressors and modern techniques for a more efficient aerodynamic design. Design and Analysis of Centrifugal Compressors isacomprehensive overview of the theoretical fluid dynamic models describing the flow in centrifugal compressors and the modern techniques for the design of more efficient centrifugal compressors. The author – a noted expert in the field, with over 40 years of experience – evaluates relevant numerical and analytical prediction models for centrifugal compressors with special attention to their accuracy and limitations. Relevant knowledge from the last century is linked with new insights obtained from modern CFD. Emphasis is to link the flow structure, performance and stability to the geometry of the different compressor components. Design and Analysis of Centrifugal Compressors is an accessible resource that combines theory with experimental data and previous research with recent developments in computational design and optimization. This important resource Covers the basic information concerning fluid dynamics that are specific for centrifugal compressors and clarifies the differences with axial compressors Provides an overview of performance prediction models previously developed in combination with extra results from research conducted by the author Describes helpful numerical and analytical models for the flow in the different components in relation to flow stability, operating range and performance Includes the fundamental information for the aerodynamic design of more efficient centrifugal compressors Explains the use of computational fluid dynamics (CFD) for the design and analysis of centrifugal compressors Written for engineers, researchers and designers in industry as well as for academics specializing in the field, Design and Analysis of Centrifugal Compressors offers an up to date overview of the information needed for the design of more effective centrifugal compressors.

The concept of digital risk, which has become ubiquitous in the media, sustains a number of myths and beliefs about the digital world. This book explores the opposite view of these ideologies by focusing on digital risks as perceived by actors in their respective contexts.

Perceptions and Analysis of Digital Risks identifies the different types of risks that concern actors and actually impact their daily lives, within education or various socio-professional environments. It provides an analysis of the strategies used by the latter to deal with these risks as they conduct their activities; thus making it possible to characterize the digital cultures and, more broadly, the informational cultures at work.

This book offers many avenues for action in terms of educating the younger generations, training teachers and leaders, and mediating risks.