Tag-based Next Generation Sequencing

Written by leading experts from industry and academia, this first single comprehensive resource addresses recent developments in next generation DNA sequencing technology and their impact on genome research, drug discovery and health care. As such, it presents a detailed comparative analysis of commercially available platforms as well as insights into alternative, emerging sequencing techniques. In addition, the book not only covers the principles of DNA sequencing techniques but also social, ethical and commercial aspects, the concept of personalized medicine and a five-year perspective of DNA sequencing.

Introduces readers to core algorithmic techniques for next-generation sequencing (NGS) data analysis and discusses a wide range of computational techniques and applications This book provides an in-depth survey of some of the recent developments in NGS and discusses mathematical and computational challenges in various application areas of NGS technologies. The 18 chapters featured in this book have been authored by bioinformatics experts and represent the latest work in leading labs actively contributing to the fast-growing field of NGS. The book is divided into four parts: Part I focuses on computing and experimental infrastructure for NGS analysis, including chapters on cloud computing, modular pipelines for metabolic pathway reconstruction, pooling strategies for massive viral sequencing, and high-fidelity sequencing protocols. Part II concentrates on analysis of DNA sequencing data, covering the classic scaffolding problem, detection of genomic variants, including insertions and deletions, and analysis of DNA methylation sequencing data. Part III is devoted to analysis of RNA-seq data. This part discusses algorithms and compares software tools for transcriptome assembly along with methods for detection of alternative splicing and tools for transcriptome quantification and differential expression analysis. Part IV explores computational tools for NGS applications in microbiomics, including a discussion on error correction of NGS reads from viral populations, methods for viral quasispecies reconstruction, and a survey of state-of-the-art methods and future trends in microbiome analysis. Computational Methods for Next Generation Sequencing Data Analysis: Reviews computational techniques such as new combinatorial optimization methods, data structures, high performance computing, machine learning, and inference algorithms Discusses the mathematical and computational challenges in NGS technologies Covers NGS error correction, de novo genome transcriptome assembly, variant detection from NGS reads, and more This text is a reference for biomedical professionals interested in expanding their knowledge of computational techniques for NGS data analysis. The book is also useful for graduate and post-graduate students in bioinformatics.

An ideal starting point for anyone wanting to learn about next generation wireless networks Gives important insights into the design of wireless IP networks Illustrates the standards and network architectures defined by leading standards bodies (including MWIF, 3GPP and 3GPP2) Discusses protocols in four key areas: signaling, mobility, quality of service, and security The authors have a good deal of experience in this field, and have many patents pending in the area of wireless networking

Next Generation Networks (NGN) provide ubiquitous connectivity with pervasive accessibility to service, application, content and information. NGN will bring tremendous advantages to companies and individuals, in terms of access to information, education and knowledge, efficiency, dematerialisation and new user experiences. Next Generation Networks: Perspectives and Potentials explores the potentials of NGN and provides an outlook of future services for the end users and opportunities for the traditional network operators and new players. It creates a framework to aid the understanding of NGN, exploring the strategic development and practical deployment of NGN. This book provides a complete and comprehensive picture of the future directions, substantial benefits, issues, applications and services for NGN. Offers an in-depth exploration of NGN covering both basic and advanced concepts Examines critical issues with the implementation of NGN Covers NGN technology, architecture, transport, services, and evolution and standardization. Written by industry experts focusing on the business opportunities of NGN with chapters on NGN standardization, development and corporate responsibility Next Generation Networks is ideal for network operators, equipment vendors, researchers, Telecoms regulators and engineers working in next generation networking. It will also be of interest to graduate students on electrical engineering and computer science programmes with a focus on networks.

What will the future of wireless communications look like? What drives mobile communications systems beyond 3G? In Next Generation Mobile Systems the authors answer these questions and others surrounding the new technologies. The book examines the current research issues driving the wireless world and provides an inclusive overview of how established technologies will evolve to suit next generation mobile systems. While the term ‘4G’ already dominates research in industry and academia, there are still numerous hurdles to take before this ambitious concept can become reality. Acclaimed researchers from NTT-DoCoMo take up the debate of what type of mobile communications will emerge in the post-3G era. Next Generation Mobile Systems: Covers the evolution of IP-based systems and IP mobility. Gives a detailed overview of radio-access technologies and wireless LANs. Explains APIs for mobile systems and IP mobility. Addresses middleware and applications, including terminal platform technologies, multimedia, and wireless web services. Discusses security in future mobile networks, including sections on Cryptographic Algorithms and Protocols for XG, Authentication, Authorization, and Accounting, and Security Policy Enforcement for Downloaded Code. This valuable resource will provide communications engineers, telecommunications managers and researchers in industry and academia with a sound understanding of the future direction of mobile technology.

Cuts through the hype! Golding's compelling offers visionary, but practical insights. A must have" reference treatment for all practitioners in the mobile innovation space." —Jag Minhas, Telef?nica O2 Europe Second edition of this best-selling guide to Wireless Applications: fully revised, updated and with brand new material! In Next Generation Wireless Applications, Second Edition, the author establishes a picture of the entire mobile application ecosystem, and explains how it all fits together. This edition builds upon the successes of the first edition by offering an up-to-date holistic guide to mobile application development, including an assessment of the applicability of new mobile applications, and an exploration into the developments in a number of areas such as Web 2.0, 3G, Mobile TV, J2ME (Java 2 Micro Edition) and many more. Key features of this second edition include: New introductory chapters on trends in mobile application, and on becoming an Operator. Two new chapters on Mobile 2.0 and IMS and Mobilizing Media and TV. Extra material on convergence, Web 2.0, AJAX (Asynchronous JavaScript and XML), HSDPA (High Speed Downlink Packet Access) and MBMS (Multimedia Broadcast Multicast Service), WiMAX (Worldwide Interoperability for Microwave Access) and WiFi. Best practice on how to present to, sell to and work with operators. More insights, anecdotes and sidebars reflecting the author's extensive experience in the industry. Next Generation Wireless Applications will prove essential reading for professionals in mobile operator and mobile application developing companies, web developers, and developer community managers. Media companies, general managers, business analysts, students, business consultants, and Java developers will also find this book captivating. «If you want to understand the future of mobile applications and services, their potential impact and the growth opportunities this is the perfect starting point.» —Martin Smith, Head of Content Innovation & Applications, T-Mobile

Future wireless communication systems should be operating mainly, if not completely, on burst data services carrying multimedia traffic.The need to support high-speed burst traffic has already posed a great challenge to all currently available air-link technologies based either on TDMA or CDMA.The first generation CDMA technology has been used in both 2G and 3G mobile cellular standards and it has been suggested that it is not suitable for high-speed burst-type traffic. There are many problems with the first generation CDMA technology, such as its low spreading efficiency, interference-limited capacity and the need for precision power control, etc… 'The Next Generation Technologies' will offer first-hand information on how to make use of various innovative technologies to implement the next generation CDMA technology. As an all-in-one reference for telecommunications engineers, advanced R & D personnels, undergraduate and postgraduate students, this book is must-read material. Addresses various important issues about the next generation CDMA technologies as the major air-link technology for beyond 3G wireless applications. Covers topics from next generation CDMA system modelling to analytical methodology, starting with the basics and progressing to advanced research topics. Contains many new and previously unpublished research results. Introduces many innovative CDMA technologies such as DS/CC-CDMA, OS/CC-CDMA, space-time complementary coding CDMA, M-ary CDMA, optical complementary coded CDMA, etc.

This is a pioneering textbook on the comprehensive description of AeroMACS technology. It also presents the process of developing a new technology based on an established standard, in this case IEEE802.16 standards suite. The text introduces readers to the field of airport surface communications systems and provides them with comprehensive coverage of one the key components of the Next Generation Air Transportation System (NextGen); i.e., AeroMACS. It begins with a critical review of the legacy aeronautical communications system and a discussion of the impetus behind its replacement with network-centric digital technologies. It then describes wireless mobile channel characteristics in general, and focuses on the airport surface channel over the 5GHz band. This is followed by an extensive coverage of major features of IEEE 802.16-2009 Physical Layer (PHY)and Medium Access Control (MAC) Sublayer. The text then provides a comprehensive coverage of the AeroMACS standardization process, from technology selection to network deployment. AeroMACS is then explored as a short-range high-data-throughput broadband wireless communications system, with concentration on the AeroMACS PHY layer and MAC sublayer main features, followed by making a strong case in favor of the IEEE 802.16j Amendment as the foundational standard for AeroMACS networks. AeroMACS: An IEEE 802.16 Standard-Based Technology for the Next Generation of Air Transportation Systems covers topics such as Orthogonal Frequency Division Multiple Access (OFDMA), coded OFDMA, scalable OFDMA, Adaptive Modulation-Coding (AMC), Multiple-Input Multiple-Output (MIMO) systems, Error Control Coding (ECC) and Automatic Repeat Request (ARQ) techniques, Time Division Duplexing (TDD), Inter-Application Interference (IAI), and so on. It also looks at future trends and developments of AeroMACS networks as they are deployed across the world, focusing on concepts that may be applied to improve the future capacity. In addition, this text: Discusses the challenges posed by complexities of airport radio channels as well as those pertaining to broadband transmissions Examines physical layer (PHY) and Media Access Control (MAC) sublayer protocols and signal processing techniques of AeroMACS inherited from IEEE 802.16 standard and WiMAX networks Compares AeroMACS and how it relates to IEEE 802.16 Standard-Based WiMAX AeroMACS: An IEEE 802.16 Standard-Based Technology for the Next Generation of Air Transportation Systems will appeal to engineers and technical professionals involved in the research and development of AeroMACS, technical staffers of government agencies in aviation sectors, and graduate students interested in standard-based wireless networking analysis, design, and development.

Since the turn of the twentieth century, telecommunications has shifted from traditional voice transport to data transport, although digitized voice is still a large contributor. Instead of an evolution of existing transport standards, a revolution was necessary in order to enable additional data-related transport. Next Generation SDH/SONET provides a detailed description of the enablers of efficient data transport over any synchronous network. These include virtual concatenation (VCAT), the operation to provide more granularity, and the link capacity adjustment scheme (LCAS), an extension of VCAT that provides more flexibility. Equally, generic framing procedure (GFP), the methodology that efficiently transports asynchronous, or variable bit-rate data signals over a synchronous or constant bit-rate, is explored in detail. Describes new extensions to SDH/SONET standards to provide more granularity and flexibility in their structures, enabling the efficient transport of data-related signals such as Ethernet and FICON Presents comprehensive sections on the implementation of multi-service transport platforms (MSTP) enabled by VCAT, LCAS and GFP Provides valuable advice on how to exploit existing networks to create or extend LANs towards metro (MAN) or wide (WAN) area networks and also to support storage area (SAN) networks This volume will appeal to manufacturers, engineers and all those involved in developing and deploying SDH, SONET and OTN technology. It will also be an invaluable resource for postgraduate students on network communications courses.

This book aims to extract the «molecular genes» leading to craziness! Geniuses are the ones who are «crazy enough to think they can change the world» and boldly go where no one has gone before. Where no past habit and usage are available, there is no proof of viability, as nobody has done it yet, or even imagined it, and no roadmap for guidance or market study has come up with it. The authors call upon Leonardo Da Vinci, the Renaissance genius, who as strange as it seems, shared many traits of personality with that of Steve Jobs, in terms of the ways of performing. Da Vinci helps in understanding Jobs, and hence Apple, with his unique way of designing radically novel concepts, which were actually quite crazy for his time. In order to shed light on a special creative posture, the indomitable sense of specifying undecidable objects – a hallmark of the late Steve Jobs – is what led the authors to match it with a specific design innovation theory. A real theory, backed by solid mathematical proof, exists and can account for the business virtue of a prolific ability to move into unknown crazy fields! The authors postulate that, by bringing the power of C-K theory to crack open a number of previous observations made about Apple’s methods, it is possible to identify most of the genes of this company. The authors analyze how and why an Apple way of doing business is radically different from standard business practices and why it is so successful. Genes are a measure of the entity at hand and can encourage past business education routine approaches, then become transferable across the spectrum of the socio-economic world.