Detection and Quantification of Antibodies to Biopharmaceuticals. Practical and Applied Considerations

A guide to state-of-the-art molecular tools for monitoring and managing the toxigenicity of cyanobacteria Runaway eutrophication and climate change has made the monitoring and management of toxigenic organisms in the world’s bodies of water more urgent than ever. In order to influence public policy regarding the detection and quantification of those organisms, it is incumbent upon scientists to raise the awareness of policy makers concerning the increased occurrence of toxigenic cyanobacteria and the threats they pose. As molecular methods can handle many samples in short time and help identify toxigenic organisms, they are reliable, cost-effective tools available for tracking toxigenic cyanobacteria worldwide. This volume arms scientists with the tools they need to track toxigenicity in surface waters and food supplies and, hopefully, to develop new techniques for managing the spread of toxic cyanobacteria. This handbook offers the first comprehensive treatment of molecular tools for monitoring toxigenic cyanobacteria. Growing out of the findings of the landmark European Cooperation in Science and Technology Cyanobacteria project (CYANOCOST), it provides detailed, practical coverage of the full array of available molecular tools and protocols, from water sampling, nucleic acid extraction, and downstream analysis—including PCR and qPCR based methods—to genotyping (DGGE), diagnostic microarrays, and community characterization using next-gen sequencing techniques. Offers an overview of the latest trends in the field, while providing a foundation for understanding and applying the tools and techniques described Provides detailed coverage of the full range of molecular tools currently available, with expert guidance on the analysis and interpretation of results Includes step-by-step guidance on standard operational procedures, including molecular tests used in environmental monitoring, with individual chapters devoted to each procedure Complements the published Handbook of Cyanobacterial Monitoring and Cyanotoxin Analysis from the CyanoCOST project This handbook is an indispensable working resource for scientists, lab technicians, and water management professionals and an excellent text/reference for graduate students and supervisors who use molecular tools. It will also be of great value to environmental health and protection officials and policy makers.

This book focuses on computational methods for large-scale statistical inverse problems and provides an introduction to statistical Bayesian and frequentist methodologies. Recent research advances for approximation methods are discussed, along with Kalman filtering methods and optimization-based approaches to solving inverse problems. The aim is to cross-fertilize the perspectives of researchers in the areas of data assimilation, statistics, large-scale optimization, applied and computational mathematics, high performance computing, and cutting-edge applications. The solution to large-scale inverse problems critically depends on methods to reduce computational cost. Recent research approaches tackle this challenge in a variety of different ways. Many of the computational frameworks highlighted in this book build upon state-of-the-art methods for simulation of the forward problem, such as, fast Partial Differential Equation (PDE) solvers, reduced-order models and emulators of the forward problem, stochastic spectral approximations, and ensemble-based approximations, as well as exploiting the machinery for large-scale deterministic optimization through adjoint and other sensitivity analysis methods. Key Features: • Brings together the perspectives of researchers in areas of inverse problems and data assimilation. • Assesses the current state-of-the-art and identify needs and opportunities for future research. • Focuses on the computational methods used to analyze and simulate inverse problems. • Written by leading experts of inverse problems and uncertainty quantification. Graduate students and researchers working in statistics, mathematics and engineering will benefit from this book.

The Novartis Foundation Series is a popular collection of the proceedings from Novartis Foundation Symposia, in which groups of leading scientists from a range of topics across biology, chemistry and medicine assembled to present papers and discuss results. The Novartis Foundation, originally known as the Ciba Foundation, is well known to scientists and clinicians around the world.

Written for industrial and academic researchers and development scientists in the life sciences industry, Bioprocessing Technology for Production of Biopharmaceuticals and Bioproducts is a guide to the tools, approaches, and useful developments in bioprocessing. This important guide: • Summarizes state-of-the-art bioprocessing methods and reviews applications in life science industries • Includes illustrative case studies that review six milestone bio-products • Discuses a wide selection of host strain types and disruptive bioprocess technologies

Fundamentals of Cancer Detection, Treatment, and Prevention The professional guide to cancer diagnosis and therapy for researchers and clinicians In Fundamentals of Cancer Detection, Treatment, and Prevention, distinguished researcher Surya K. De delivers a concise and authoritative guide to cancer treatment, diagnosis, and prevention. The book offers a comprehensive overview of cancer in humans, from its causes, symptoms, and diagnosis to the variety of treatment options available today. Intuitively organized by cancer type, this guide provides concise information on risk factors, diagnosis, and treatment options for all commonly encountered tumors, including surgery, radiation therapy, chemotherapy, and immunotherapy. All US FDA-approved drugs—like small molecules, peptides, monoclonal antibodies, whole antibodies, gene therapy, antibody-drug conjugates, and cell therapies—are considered, and information about their generic and brand names, clinical uses, and mechanisms of action is presented. Readers will also find: A thorough overview of human cancers, including cancer risk factors and possible preventions Comprehensive explorations of bladder, blood, brain, and spinal cord cancers Practical discussions of breast, colorectal, cervical, kidney, and liver cancer In-depth examinations of lung, skin, ovarian, vaginal, vulvar, pancreatic, and prostate cancers, as well as mesothelioma Perfect for pharmaceutical chemists, oncologists, pharmacologists, and medicinal chemists, Fundamentals of Cancer Detection, Treatment, and Prevention is an indispensable guide for professional researchers, whether they are working in the clinic or the pharmaceutical industry.

Research evidence concerning the precursors (a sign or symptom that heralds another) and prodromes (an early or premonitory symptom of a disease) of the various mental disorders is growing but is at present widely dispersed and inaccessible. Clinicians have to rely on their own experience, which is often limited, when faced with several delicate diagnostic and treatment issues. This book aims to approach for the first time in a comprehensive way this emerging area of clinical psychiatry. The World Health Organisation has found that mental disorders such as depression and psychosis rank in the top 10 of leading causes of disability in the world, creating a significant social, emotional and economic burden for young people, their families and society. Early detection of these potentially disabling disorders and treatment at the time of initial onset can greatly improve long-term outcomes and effect dramatic cost-savings. The related Birchwood title has sold nearly 2300 copies and is popular at psychiatry conferences. From an R&D perspective, industry have a stake in this area. Obviously, if biomarkers or other prodromal evidence can be determined in various disorders, then drugs will start to be dispensed earlier. The topic has increased in popularity as a subject for satellite symposia e.g Jeffrey Lieberman chaired the Bristol Myers Squibb symposia at the American Psychiatric Association. This dealt with early intervention in schizophrenia and the need for novel approaches in the prodromal phase. In addition, more symposia (and research) look at disorders «across the lifespan.» This phrase indicates an approach to research, diagnosis and treatment from a more quality-of-life vantage and early diagnosis is part of this movement.

An Introduction to Applied and Environmental Geophysics, 2nd Edition, describes the rapidly developing field of near-surface geophysics. The book covers a range of applications including mineral, hydrocarbon and groundwater exploration, and emphasises the use of geophysics in civil engineering and in environmental investigations. Following on from the international popularity of the first edition, this new, revised, and much expanded edition contains additional case histories, and descriptions of geophysical techniques not previously included in such textbooks. The level of mathematics and physics is deliberately kept to a minimum but is described qualitatively within the text. Relevant mathematical expressions are separated into boxes to supplement the text. The book is profusely illustrated with many figures, photographs and line drawings, many never previously published. Key source literature is provided in an extensive reference section; a list of web addresses for key organisations is also given in an appendix as a valuable additional resource. Covers new techniques such as Magnetic Resonance Sounding, Controlled- Source EM, shear-wave seismic refraction, and airborne gravity and EM techniques Now includes radioactivity surveying and more discussions of down-hole geophysical methods; hydrographic and Sub-Bottom Profiling surveying; and UneXploded Ordnance detection Expanded to include more forensic, archaeological, glaciological, agricultural and bio-geophysical applications Includes more information on physio-chemical properties of geological, engineering and environmental materials Takes a fully global approach Companion website with additional resources available at www.wiley.com/go/reynolds/introduction2e Accessible core textbook for undergraduates as well as an ideal reference for industry professionals The second edition is ideal for students wanting a broad introduction to the subject and is also designed for practising civil and geotechnical engineers, geologists, archaeologists and environmental scientists who need an overview of modern geophysical methods relevant to their discipline. While the first edition was the first textbook to provide such a comprehensive coverage of environmental geophysics, the second edition is even more far ranging in terms of techniques, applications and case histories.

Biotechnology and Biopharmaceuticals: Transforming Proteins and Genes into Drugs, Second Edition addresses the pivotal issues relating to translational science, including preclinical and clinical drug development, regulatory science, pharmaco-economics and cost-effectiveness considerations. The new edition also provides an update on new proteins and genetic medicines, the translational and integrated sciences that continue to fuel the innovations in medicine, as well as the new areas of therapeutic development including cancer vaccines, stem cell therapeutics, and cell-based therapies.

Biotechnology and Biopharmaceuticals: Transforming Proteins and Genes into Drugs, Second Edition addresses the pivotal issues relating to translational science, including preclinical and clinical drug development, regulatory science, pharmaco-economics and cost-effectiveness considerations. The new edition also provides an update on new proteins and genetic medicines, the translational and integrated sciences that continue to fuel the innovations in medicine, as well as the new areas of therapeutic development including cancer vaccines, stem cell therapeutics, and cell-based therapies.