Introduction to Mass Spectrometry

With contributions from noted experts from Europe and North America, Mass Spectrometry Instrumentation, Interpretation, and Applications serves as a forum to introduce students to the whole world of mass spectrometry and to the many different perspectives that each scientific field brings to its use. The book emphasizes the use of this important analytical technique in many different fields, including applications for organic and inorganic chemistry, forensic science, biotechnology, and many other areas. After describing the history of mass spectrometry, the book moves on to discuss instrumentation, theory, and basic applications.

The first systematic summary of biophysical mass spectrometry techniques Recent advances in mass spectrometry (MS) have pushed the frontiers of analytical chemistry into the biophysical laboratory. As a result, the biophysical community's acceptance of MS-based methods, used to study protein higher-order structure and dynamics, has accelerated the expansion of biophysical MS. Despite this growing trend, until now no single text has presented the full array of MS-based experimental techniques and strategies for biophysics. Mass Spectrometry in Biophysics expertly closes this gap in the literature. Covering the theoretical background and technical aspects of each method, this much-needed reference offers an unparalleled overview of the current state of biophysical MS. Mass Spectrometry in Biophysics begins with a helpful discussion of general biophysical concepts and MS-related techniques. Subsequent chapters address: * Modern spectrometric hardware * High-order structure and dynamics as probed by various MS-based methods * Techniques used to study structure and behavior of non-native protein states that become populated under denaturing conditions * Kinetic aspects of protein folding and enzyme catalysis * MS-based methods used to extract quantitative information on protein-ligand interactions * Relation of MS-based techniques to other experimental tools * Biomolecular properties in the gas phase Fully referenced and containing a helpful appendix on the physics of electrospray mass spectrometry, Mass Spectrometry in Biophysics also offers a compelling look at the current challenges facing biomolecular MS and the potential applications that will likely shape its future.

First explaining the basic principles of liquid chromatography and mass spectrometry and then discussing the current applications and practical benefits of LC-MS, along with descriptions of the basic instrumentation, this title will prove to be the indispensable reference source for everyone wishing to use this increasingly important tandem technique. * First book to concentrate on principles of LC-MS * Explains principles of mass spectrometry and chromatography before moving on to LC-MS * Describes instrumental aspects of LC-MS * Discusses current applications of LC-MS and shows benefits of using this technique in practice

Modern mass spectrometry – the instrumentation and applications in diverse fields Mass spectrometry has played a pivotal role in a variety of scientific disciplines. Today it is an integral part of proteomics and drug discovery process. Fundamentals of Contemporary Mass Spectrometry gives readers a concise and authoritative overview of modern mass spectrometry instrumentation, techniques, and applications, including the latest developments. After an introduction to the history of mass spectrometry and the basic underlying concepts, it covers: Instrumentation, including modes of ionization, condensed phase ionization techniques, mass analysis and ion detection, tandem mass spectrometry, and hyphenated separation techniques Organic and inorganic mass spectrometry Biological mass spectrometry, including the analysis of proteins and peptides, oligosaccharides, lipids, oligonucleotides, and other biological materials Applications to quantitative analysis Based on proven teaching principles, each chapter is complete with a concise overview, highlighted key points, practice exercises, and references to additional resources. Hints and solutions to the exercises are provided in an appendix. To facilitate learning and improve problem-solving skills, several worked-out examples are included. This is a great textbook for graduate students in chemistry, and a robust, practical resource for researchers and scientists, professors, laboratory managers, technicians, and others. It gives scientists in diverse disciplines a practical foundation in modern mass spectrometry.

Serves as a practical reference for those involved in Secondary Ion Mass Spectrometry (SIMS) • Introduces SIMS along with the highly diverse fields (Chemistry, Physics, Geology and Biology) to it is applied using up to date illustrations • Introduces the accepted fundamentals and pertinent models associated with elemental and molecular sputtering and ion emission • Covers the theory and modes of operation of the instrumentation used in the various forms of SIMS (Static vs Dynamic vs Cluster ion SIMS) • Details how data collection/processing can be carried out, with an emphasis placed on how to recognize and avoid commonly occurring analysis induced distortions • Presented as concisely as believed possible with All sections prepared such that they can be read independently of each other

The authoritative guide to analyzing protein interactions by mass spectrometry Mass spectrometry (MS) is playing an increasingly important role in the study of protein interactions. Mass Spectrometry of Protein Interactionspresents timely and definitive discussions of the diverse range of approaches for studying protein interactions by mass spectrometry with an extensive set of references to the primary literature. Each chapter is written by authors or teams of authors who are international authorities in their fields. This leading reference text: * Discusses the direct detection of protein interactions through electrospray ionization (ESI-MS); ion mobility analysis; and matrix-assisted laser desorption/ionization (MALDI-MS) * Covers the indirect analysis of protein interactions through hydrogen-deuterium exchange (HX-MS); limited proteolysis; cross-linking; and radial probe (RP-MS) * Guides researchers in the use of mass spectrometry in structural biology, biochemistry, and protein science to map and define the huge number and diversity of protein interactions * Reviews the latest discoveries and applications and addresses new and ongoing challenges This is a comprehensive reference for researchers in academia and industry engaged in studies of protein interactions and an excellent text for graduate and postgraduate students.

A definitive reference, completely updated Published in 1989, the First Edition of this book, originally entitled Quadrupole Storage Mass Spectrometry, quickly became the definitive reference in analytical laboratories worldwide. Revised to reflect scientific and technological advances and new applications in the field, the Second Edition includes new chapters covering: * New ion trap instruments of high sensitivity * Peptide analysis by liquid chromatography/ion trap tandem mass spectrometry * Analytical aspects of ion trap mass spectrometry combined with gas chromatography * Simulation of ion trajectories in the ion trap One additional chapter discusses the Rosetta mission, a «comet chaser» that was sent on a ten-year journey in 2004 to study the comet Churyumov-Gerasimenko using, among other instruments, a GC/MS system incorporating a specially designed ion trap mass spectrometer. This comprehensive reference also includes discussions of the history of the quadrupole ion trap, the theory of quadrupole mass spectrometry, the dynamics of ion-trapping chemistry in the quadrupole ion trap, the cylindrical ion trap, miniature traps, and linear ion traps. Complete with conclusions and references, this primer effectively encapsulates the body of knowledge on quadrupole ion trap mass spectrometry. With its concise descriptions of the theory of ion motion and the principles of operation, Quadrupole Ion Trap Mass Spectrometry, Second Edition is ideal for new users of quadrupole devices, as well as for scientists, researchers, and graduate and post-doctoral students working in analytical laboratories.

This first book to discuss both separation chemistry and mass spectrometry for mineral and rock analysis compares the two frequently used techniques, analyzing both their scope and limitations by way of numerous practical examples. The excellent and highly experienced author adopts a comprehensive and systematic approach, reviewing all the steps involved in an analytical workflow. In addition to thermal ionization mass spectrometry (TIMS), he also discusses applications of ICP-MS. Furthermore, alongside detailed protocols on sample preparation and mass spectrometric measurements, numerous practical hints are given. A must-have handy guide for all isotope geochemists and anyone involved in isotope analysis.

Presents Practical Applications of Mass Spectrometry for Protein Analysis and Covers Their Impact on Accelerating Drug Discovery and Development Covers both qualitative and quantitative aspects of Mass Spectrometry protein analysis in drug discovery Principles, Instrumentation, Technologies topics include MS of peptides, proteins, and ADCs , instrumentation in protein analysis, nanospray technology in MS protein analysis, and automation in MS protein analysis Details emerging areas from drug monitoring to patient care such as Identification and validation of biomarkers for cancer, targeted MS approaches for biomarker validation, biomarker discovery, and regulatory perspectives Brings together the most current advances in the mass spectrometry technology and related method in protein analysis

Explores the impact of the latest breakthroughs in cluster SIMS technology Cluster secondary ion mass spectrometry (SIMS) is a high spatial resolution imaging mass spectrometry technique, which can be used to characterize the three-dimensional chemical structure in complex organic and molecular systems. It works by using a cluster ion source to sputter desorb material from a solid sample surface. Prior to the advent of the cluster source, SIMS was severely limited in its ability to characterize soft samples as a result of damage from the atomic source. Molecular samples were essentially destroyed during analysis, limiting the method's sensitivity and precluding compositional depth profiling. The use of new and emerging cluster ion beam technologies has all but eliminated these limitations, enabling researchers to enter into new fields once considered unattainable by the SIMS method. With contributions from leading mass spectrometry researchers around the world, Cluster Secondary Ion Mass Spectrometry: Principles and Applications describes the latest breakthroughs in instrumentation, and addresses best practices in cluster SIMS analysis. It serves as a compendium of knowledge on organic and polymeric surface and in-depth characterization using cluster ion beams. It covers topics ranging from the fundamentals and theory of cluster SIMS, to the important chemistries behind the success of the technique, as well as the wide-ranging applications of the technology. Examples of subjects covered include: Cluster SIMS theory and modeling Cluster ion source types and performance expectations Cluster ion beams for surface analysis experiments Molecular depth profiling and 3-D analysis with cluster ion beams Specialty applications ranging from biological samples analysis to semiconductors/metals analysis Future challenges and prospects for cluster SIMS This book is intended to benefit any scientist, ranging from beginning to advanced in level, with plenty of figures to help better understand complex concepts and processes. In addition, each chapter ends with a detailed reference set to the primary literature, facilitating further research into individual topics where desired. Cluster Secondary Ion Mass Spectrometry: Principles and Applications is a must-have read for any researcher in the surface analysis and/or imaging mass spectrometry fields.